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General Information

Course ID (CB01A and CB01B)
HTEC D085B
Course Title (CB02)
Clinical Chemistry II Laboratory
Course Credit Status
Credit - Degree Applicable
Effective Term
Fall 2023
Course Description
Teaches instrumentation methodologies and operations used in basic clinical chemistry analysis. The student will perform method verification studies required by clinical laboratories. Variables of the pre-analytical, analytical, and post-analytical phases will be covered. Characteristics important to quality lab technique and safety will be done. Correlating test results with disease states will be accomplished. Successful completion of this course, HTEC D085B, and HTEC D085D are required to enroll in Clinical Chemistry Practicum, HTEC D185.
Faculty Requirements
Course Family
Not Applicable

Course Justification

This course is CSU transferable and part of a CTE program. This is a course that was developed based on the National Accrediting Agency of Clinical Laboratory Sciences and California State Department of Public Health accreditation standards required for Medical Laboratory Technicians' training programs. This course belongs on the Certification of Proficiency-Advanced and an Associates degree in Medical Laboratory Technology. This course is designed to introduce students to advanced instrumentation and methodologies used in the chemistry department of a clinical laboratory.

Foothill Equivalency

Does the course have a Foothill equivalent?
No
Foothill Course ID

Course Philosophy

Formerly Statement

Course Development Options

Basic Skill Status (CB08)
Course is not a basic skills course.
Grade Options
  • Letter Grade
  • Pass/No Pass
Repeat Limit
0

Transferability & Gen. Ed. Options

Transferability
Transferable to CSU only

Units and Hours

Summary

Minimum Credit Units
1.5
Maximum Credit Units
1.5

Weekly Student Hours

TypeIn ClassOut of Class
Lecture Hours0.00.0
Laboratory Hours4.50.0

Course Student Hours

Course Duration (Weeks)
12.0
Hours per unit divisor
36.0
Course In-Class (Contact) Hours
Lecture
0.0
Laboratory
54.0
Total
54.0
Course Out-of-Class Hours
Lecture
0.0
Laboratory
0.0
NA
0.0
Total
0.0

Prerequisite(s)

HTEC D085A

Corequisite(s)

HTEC D085D

Advisory(ies)

Limitation(s) on Enrollment

Entrance Skill(s)

Students must read, agree and submit the essential form. This form states that you meet the basic skills needed to successfully perform laboratory testing. Forms can be found on the MLT website.

General Course Statement(s)

Methods of Instruction

Use of visual aids

Discussion of assigned reading

Discussion and problem solving performed in class

Quiz and examination review performed in class

Field observation and field trips

Collaborative learning and small group exercises

Laboratory experience which involve students in formal exercises of data collection and analysis

Laboratory discussion sessions and quizzes that evaluate the proceedings weekly laboratory exercises

Assignments

  1. Readings from the required text, laboratory manual and supplemental sources.
  2. Complete laboratory worksheets that include observations, experimental results and critical analysis of data.
  3. Perform laboratory procedures as outlined in the laboratory manual.

Methods of Evaluation

  1. Class activity - Discussions and oral question and answer sessions that test comprehension and require synthesis and application of course material.
  2. Lab activity - Practice and demonstration of techniques in the student laboratory designed to demonstrate critical thinking skills and to problem solve as required in the assignments and experimental investigations.
  3. Written Assignments - Laboratory worksheets requiring the student to apply technical concepts taught in this class to given situations and scenarios.
  4. Exams
  5. Objective test - Written test examination requiring the student to apply technical and theoretical concepts taught in this class to given situations and scenarios.
  6. Quizzes - Quizzes designed to cover each laboratory session will require the student to apply technical aspects of recently presented course material on a routine basis and help identify any areas that may need extra attention.
  7. Comprehensive Final Exam - Written test and hands on demonstration of proper laboratory technique requiring the student to demonstrate their ability to summarize, integrate and critically analyze concepts examined throughout the course.
  8. Problem solving - Case studies and analyzing unknown samples evaluate the students ability to apply critical thinking skills to a clinical situation.
  9. Skill Demonstration - Laboratory practical examinations demonstrating the student's ability to integrate the knowledge acquired in the course with the technical skills necessary for the MLT profession.

Essential Student Materials/Essential College Facilities

Essential Student Materials
  • Computer with internet access.
Essential College Facilities
  • Laboratory classroom equipped with automated instrumentation and general supplies to accommodate a Clinical Chemistry II laboratory

Examples of Primary Texts and References

AuthorTitlePublisherDate/EditionISBN
Bishop, Michael L., Fody, Edward P;, Van Siclen, Carleen, Mistler, James March, Moy, MichelleClinical Chemistry Principles, Techniques, and CorrelationsJones & Bartlett Learning9th Edition13 978-1496335586
Doucette, Lorraine J.Mathematics for the Clinical LaboratoryElsevier978-0-323-55482-4

Examples of Supporting Texts and References

AuthorTitlePublisher
Burtis, Carl, Edward Ashwood, David Bruns, and Barbara Sawyer. "Tietz, Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th edition". St. Louis: Elsevier Science, 2014.
Sunheimer, Robert L. and Linda Graves. "Clinical Laboratory Chemistry, First Edition". Upper Saddle River: Prentice Hall, 2010.
Turgeon, Mary Louise. "Clinical Laboratory Science, 6th edition". St. Louis: Elsevier Science, 2011.

Learning Outcomes and Objectives

Course Objectives

  • Practice the use of Standard Precautions as they apply in the chemistry laboratory according to Occupational Health and Safety Administration (OSHA) mandates.
  • Demonstrate safe use and disposal of biohazardous materials in each laboratory session.
  • Recall through demonstration correct pipetting techniques and dilution preparation.
  • Familiarize yourself with common terms used in conjunction with Laboratory Information Systems (LIS).
  • Demonstrate proficiency in the operation of automated instrumentation available in the student laboratory.
  • Perform Method Validation studies as assigned on student laboratory instrumentation
  • Explain the proteins assayed in the clinical lab, their common methods of analysis and clinical significance.
  • Perform different drug of abuse testing on kits available in the student laboratory

CSLOs

  • Practice proper application of OSHA standards as appropriate in a clinical chemistry laboratory.

  • Use troubleshooting skills to identify potential errors in laboratory testing.

Outline

  1. Practice the use of Standard Precautions as they apply in the chemistry laboratory according to Occupational Health and Safety Administration (OSHA) mandates.
    1. Demonstrate during each laboratory session, the basic aspects of infection control policies, including the use of personal protective equipment (PPE) or devices (gown, gloves, and goggles).
    2. Select, prepare and use proper disinfectants to decontaminate the work area when a hazardous spill has occurred or when beginning or ending a laboratory session.
    3. Review the location, description and/or explaination of the following:
      1. Evacuation routes
      2. Biohazardous material
      3. Blood borne pathogens
      4. Standard Precautions
      5. Aerosols
      6. MSDS (Material Safety Data Sheets)
  2. Demonstrate safe use and disposal of biohazardous materials in each laboratory session.
    1. Describe how to properly segregate and dispose of various types of waste products generated in the clinical laboratory, including the use of sharp containers for needles, lancets and/or other sharps.
    2. Demonstrate, via weekly performance, proper disposal of biological samples, as instructed by procedure or instructor.
  3. Recall through demonstration correct pipetting techniques and dilution preparation.
    1. Demonstrate, by performance, proper pipetting techniques by doing a serial dilution, two fold - 10 tubes long. Calculate the initial concentration and the concentration of the last tube.
    2. Perform other dilutions as assigned.
  4. Familiarize yourself with common terms used in conjunction with Laboratory Information Systems (LIS).
    1. Describe how the LIS system is used to input patient demographics, order tests, utilize worksheets and pending logs.
    2. Locate laboratory results, normal ranges, critical values and report verification documentation on an LIS printout.
  5. Demonstrate proficiency in the operation of automated instrumentation available in the student laboratory.
    1. Identify components and list their function in assigned laboratory instrumentation.
    2. State the normal value or therapeutic range for each assay you perform.
    3. Perform and record daily maintenance.
    4. Reconstitute controls and calibrators as assigned.
    5. Perform assay calibration and QC.
    6. Perform patient testing and correlate patient test results with possible disease states.
    7. Demonstrate steps to follow when troubleshooting an instrument.
    8. Replace components of student laboratory instrumentation as instructed.
      1. Demonstrate appropriate documentation after component replacement.
      2. Describe steps to follow when repair of replacement is unsuccessful
    9. Perform instrument troubleshooting on student laboratory instrumentation as appropriate.
      1. Document steps involved in troubleshooting
      2. Verify that you were able to "fix" the problem or determine next steps.
  6. Perform Method Validation studies as assigned on student laboratory instrumentation
    1. Record data generated on class excel sheet
    2. Analyze data and draw conclusions for each study performed
    3. Present data and conclusions in Lab Notebook
  7. Explain the proteins assayed in the clinical lab, their common methods of analysis and clinical significance.
    1. Perform electrophoresis testing on assigned samples.
    2. List the specimen consideration, interference and reference range for the electrophoretic techniques studied
    3. Evaluate given electrophoretic patterns, suggest the abnormality
  8. Perform different drug of abuse testing on kits available in the student laboratory
    1. Perform drug of abuse testing on controls and patient samples
    2. Record and interpret results of your testing and controls
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